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3.5 Nucleic Acids β Test 1
Q1. The most common configuration of amino acids and DNA respectively is:β L and B
Q2. If E. coli cells are grown in medium with radioactive Β³Β²P, the Β³Β²P would be found in all EXCEPT:β Carbohydrates
Q3. Which of the following covalent bonds exist in the structure of ATP?β Phosphoanhydride, phosphomonoester, N-glycosidic
Q4. ATP is a free nucleotide containing:β Ribose sugar
Q5. Which statement is true about base-stacking interactions in DNA? They are:β van der Waals interactions
Q6. Which molecule binds with greater affinity to a calcium hydroxyapatite column?β ds-DNA
Q7. Cytosine on deamination produces:β Uracil
Q8. Deamination of cytosine leads to the formation of:β Uracil
Q9. The sugar component of DNA is:β Ξ²-2-Deoxy-D-ribose
Q10. The sugar component of DNA is (select the correct anomer and series):β Ξ²-2-Deoxy-D-ribose
Q11. The propeller twist in the B-form of DNA is approximately:β 30Β°
Q12. Which statement is TRUE for DNA?β Can hybridise with other DNA and RNA
Q13. Which one of the following statements is true for DNA?β It can hybridise with other DNA and RNA molecules
Q14. A DNA preparation with a 260:280 nm absorbance ratio below 1.8 is most likely contaminated with:β Protein
Q15. Supercoiled DNA is soluble in:β Buffer-saturated phenol
Q16. Which of the following is NOT true about DNA?β A-DNA is usually a left-handed helix
Q17. Which one of the following is NOT a naturally occurring nucleotide?β ddATP
Q18. What atoms are linked in the N-Ξ²-glycosidic linkage of the adenine base with the sugar?β N-9 of adenine with C-1' of sugar
3.5 Nucleic Acids β Test 2
Q19. In a nucleotide, the nitrogenous base is attached to the sugar at the:β 1' carbon
Q20. The acidic character of DNA and RNA is due to:β Phosphate group
Q21. In terms of thermal stability, which order is correct?β dsDNA > dsRNA > DNA-RNA > ssDNA
Q22. Which one of the following is a nucleotide?β Cytidylic acid
Q23. Which organic compound is composed of a nitrogenous base, a single five-carbon sugar, and a triphosphate group?β Nucleotide
Q24. DNA and RNA are acidic due to the presence of:β Phosphate groups
Q25. Phosphoric acid has three pKa values. How many pKa values does a phosphate group within the DNA/RNA backbone retain?β 1
Q26. The formation of a phosphodiester bond between two nucleotides is a:β Condensation between 3'-OH and 5'-phosphate
Q27. PolydT (deoxythymidylic acid) is:β A nucleic acid
Q28. RNA is a polymer of:β Ribonucleotides
Q29. Ribose sugar is present in:β RNA and ATP
Q30. Which of the following is a monomer in RNA?β Adenosine monophosphate
Q31. Which of the following are monomers in RNA?β Adenosine monophosphate
Q32. The structural difference between uracil (RNA) and thymine (DNA) is that thymine has an extra:β Methyl group
Q33. Which of the following is the most common modification on mRNA molecules?β N6-methyladenosine (m6A)
Q34. What activities are associated with RNA enzymes (ribozymes)?β Nuclease and polymerase activity
Q35. Which one of the following has a catalytic RNA?β Ribozyme
Q36. The primary function of the hammerhead ribozyme is:β Cleavage of RNA phosphodiester bonds