2.1 Nucleotide Sequence Databases — Test 1

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2.1 Nucleotide Sequence Databases — Test 1

Q1. GenBank, the primary nucleotide sequence database, is maintained by the:✓ NCBI (USA)

Q2. The European nucleotide database, EMBL-Bank, is hosted by the:✓ EBI (European Bioinformatics Institute)

Q3. DDBJ, the third major nucleotide database, is based in:✓ Japan

Q4. GenBank, EMBL and DDBJ exchange data daily as part of the:✓ International Nucleotide Sequence Database Collaboration (INSDC)

Q5. A primary (sequence) database is one that contains:✓ Original experimental data submitted directly by researchers

Q6. A secondary database differs from a primary one in that it contains:✓ Curated or derived information analysed from primary data

Q7. Each sequence record in GenBank is uniquely identified by its:✓ Accession number

Q8. The NCBI search and retrieval system that lets users query its many databases is called:✓ Entrez

Q9. The NCBI RefSeq collection is best described as a set of:✓ Curated, non-redundant reference sequences

Q10. The Sequence Read Archive (SRA) primarily stores:✓ Raw sequencing reads from next-generation sequencing

Q11. dbEST is a division of GenBank that stores:✓ Expressed sequence tags (ESTs)

Q12. Nucleotide sequence databases such as GenBank primarily store:✓ DNA and RNA sequences with associated annotation

Q13. Ensembl and the UCSC Genome Browser are examples of:✓ Genome databases/browsers that display annotated genomes

Q14. The fact that a sequence submitted to DDBJ also becomes available in GenBank and EMBL is due to:✓ Daily data exchange within the INSDC

Q15. A version number attached to a sequence record indicates:✓ That the sequence has been updated/revised

Q16. Compared with archival GenBank, RefSeq is valued for being:✓ Non-redundant and curated

Q17. The primary purpose of submitting sequences to a public database is to:✓ Make data freely available and citable for the research community

Q18. The data in primary nucleotide databases are best described as:✓ Archival — preserving what was submitted, even if later corrected elsewhere

Q19. A GenBank record's FEATURES section provides:✓ Annotation of biological features such as genes and coding regions

Q20. Match each nucleotide database/resource with its description and select the correct option.✓ A-iii, B-i, C-iv, D-ii