Quick revision: every question with its correct answer. For the full explanation, open the test and tap View Solution.
3.4 Molecular Markers & QTL Mapping — Test 1
Q1. A RAPD (Random Amplified Polymorphic DNA) marker is:✓ Dominant
Q2. Which molecular marker uses a combination of BOTH restriction enzyme digestion and PCR?✓ AFLP
Q3. The correct statement about the AFLP technique is:✓ Restriction digestion followed by two PCR steps (pre-selective and selective)
Q4. Which molecular marker can clearly distinguish a homozygote from a heterozygote (co-dominant)?✓ RFLP
Q5. The polymorphic DNA fragment most suitable for genetic mapping is one that is:✓ Co-dominant
Q6. Simple sequence repeat (SSR / microsatellite) markers are:✓ Co-dominant
Q7. The most abundant type of DNA polymorphism in genomes is the:✓ Single nucleotide polymorphism (SNP)
Q8. RFLP detection of a specific genomic fragment is based on:✓ Hybridisation with a labelled probe (Southern blot)
Q9. DNA fingerprinting (as first developed by Alec Jeffreys) relies on minisatellites, which are:✓ Repetitive non-coding (VNTR) sequences
Q10. For tagging Tobacco Mosaic Virus (TMV) resistance in tobacco, the simplest, most economical and least time-consuming marker is:✓ RAPD
Q11. QTL (Quantitative Trait Locus) analysis is used to:✓ Identify chromosome regions associated with a complex (quantitative) trait in a genetic cross
Q12. How many genotypes can exist at a single locus with 5 alleles in a diploid organism? [use n(n+1)/2]✓ 15
Q13. An ideal mapping population for a self-pollinating crop is generated using:✓ Polymorphic inbred (pure-line) parents
Q14. Bulked segregant analysis (BSA) is mainly used to:✓ Rapidly find markers linked to a target gene/trait using pooled DNA
Q15. The coefficient of coincidence is calculated as:✓ Observed ÷ expected double crossovers
Q16. Interference in crossing over is defined as:✓ 1 − coefficient of coincidence
Q17. A LOD score of 3 or more is conventionally taken as the threshold to:✓ Declare significant linkage
Q18. Map functions such as Haldane's and Kosambi's are used to:✓ Convert recombination frequency to true map distance (correcting for multiple crossovers)
Q19. Markers based on differences in restriction sites that alter fragment length are called:✓ RFLP
Q20. Match each molecular marker with its property and select the correct option.✓ A-ii, B-i, C-iv, D-iii