Linkage, Recombination & Molecular Markers

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Welcome! 3.6 Linkage, Recombination & Molecular Markers β€” Test 2 — 20 questions, CSIR-NET style.

What this test covers

  • Crossover frequency, map distance & linkage groups
  • Recombination frequency calculations & the 50% limit
  • LOD scores, molecular markers (RFLP, SSR)
  • Somatic-cell hybrids & Neurospora centromere mapping

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3.6 Linkage, Recombination & Molecular Markers β€” Test 2
Q1. The frequency of crossing over between two genes is directly proportional to the:βœ“ Distance between the genes
Q2. In a genetic map, the distance between two genes is measured in:βœ“ Centimorgans (map units)
Q3. The maximum recombination frequency that can be observed between two loci is:βœ“ 50%
Q4. A genetic map built from recombination-frequency data is called a:βœ“ Linkage map
Q5. The number of linkage groups in a human male is:βœ“ 24
Q6. If an organism has 12 linkage groups, its haploid chromosome number is:βœ“ 12
Q7. After meiosis, 20% of the gametes are recombinant for two genes. The map distance between them is:βœ“ 20 cM
Q8. Genes A and B are 10 cM apart. In a test cross of AB/ab (cis), the proportion of AB/ab (parental) offspring of that type is about:βœ“ 45%
Q9. A two-point test cross of two UNLINKED genes gives a phenotypic ratio of:βœ“ 1:1:1:1
Q10. The ratio of the observed number of double crossovers to the expected number is the:βœ“ Coefficient of coincidence
Q11. Recombination does NOT occur during meiosis in:βœ“ Drosophila males
Q12. Which molecular marker is NON-PCR-based?βœ“ RFLP
Q13. A LOD score of 3 corresponds to odds in favour of linkage of:βœ“ 1000 to 1
Q14. The most suitable marker class for marker-assisted selection of agronomic traits is generally:βœ“ EST-SSR (expressed-sequence microsatellites)
Q15. Interspecific somatic-cell hybrids (e.g. human-mouse) have been especially useful for:βœ“ Physical gene mapping (assigning genes to chromosomes)
Q16. The fusion of human and mouse cells to make somatic-cell hybrids is commonly mediated by:βœ“ Inactivated Sendai virus (or PEG)
Q17. A molecular marker generally CANNOT be used directly for:βœ“ Identifying the expressed protein product
Q18. Neurospora is an ideal organism for determining whether allele segregation occurred at the first or second meiotic division because it:βœ“ Retains the ordered products of meiosis in an ascus
Q19. In Neurospora, second-division segregation patterns in the ascus are used to measure the distance between a gene and its:βœ“ Centromere
Q20. Match each term with its description and select the correct option.βœ“ A-ii, B-iv, C-i, D-iii