8.2 Transcription & RNA Processing — Test 1

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8.2 Transcription & RNA Processing — Test 1

Q1. During transcription-associated supercoiling in bacteria:✓ Gyrase introduces negative supercoils ahead/behind; topoisomerase I relaxes them

Q2. Eukaryotic transcription is carried out by:✓ DNA-dependent RNA polymerase

Q3. The enzyme that transcribes the large pre-rRNA (28S, 18S, 5.8S) in eukaryotes is:✓ RNA polymerase I

Q4. Which RNA is transcribed by RNA polymerase III in eukaryotes?✓ tRNA

Q5. The TATA box functions as a:✓ Core-promoter element for RNA polymerase/general factors

Q6. The 5' cap of eukaryotic mRNA contains a:✓ Modified guanine (7-methylguanosine)

Q7. The covalent linkage between the m⁷G cap and the rest of the mRNA is a:✓ 5'-5' triphosphate bond

Q8. The 5' ends of mature prokaryotic mRNAs and tRNAs are, respectively:✓ Triphosphate (mRNA) and monophosphate (tRNA)

Q9. If the DNA template strand reads 5'-AGCTTCGA-3', the mRNA synthesised is:✓ 5'-UCGAAGCU-3'

Q10. A DNA template triplet reads 5'-AGT-3'. The corresponding mRNA codon is:✓ 3'-UCA-5'

Q11. If the sense (coding) strand of DNA is 5'-ATGGACCAGA-3', the RNA produced is:✓ 5'-AUGGACCAGA-3'

Q12. The Shine-Dalgarno sequence of eubacterial mRNA is recognised by the anti-Shine-Dalgarno region of:✓ 16S rRNA

Q13. Small nucleolar RNAs (snoRNAs) primarily function in:✓ Processing and chemical modification of rRNA

Q14. Long non-coding RNAs (lncRNAs) can regulate gene expression by, among other things:✓ Modulating chromatin, transcription and miRNA activity

Q15. A riboswitch controls gene expression by:✓ Sensing a metabolite via a structured non-coding mRNA region

Q16. In eukaryotic genomes, a single protein domain is most often encoded by a single:✓ Exon

Q17. Which part of a typical eukaryotic gene is likely to be the LEAST conserved in evolution?✓ Intron

Q18. Pre-mRNA splicing (intron removal) is carried out by the:✓ Spliceosome (snRNPs)

Q19. The 3' end of most eukaryotic mRNAs is modified by addition of a:✓ Poly(A) tail

Q20. Match each eukaryotic RNA polymerase / feature with its product/role and select the correct option.✓ A-iii, B-i, C-ii, D-iv