Replication, Transcription & the Genetic Code

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Welcome! 8.4 Replication, Transcription & the Genetic Code โ€” Test 2 — 20 questions, CSIR-NET style.

What this test covers

  • DNA base pairing & semiconservative replication
  • Leading/lagging strands & primer removal
  • Genetic code: sense codons, AUG, decoding
  • Operon regulation, termination & mitochondrial genome

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8.4 Replication, Transcription & the Genetic Code โ€” Test 2
Q1. In the DNA double helix, the bases pair as:โœ“ A-T and G-C
Q2. DNA replication is best described as:โœ“ Semiconservative
Q3. Which of the following is a step in DNA replication?โœ“ All of these
Q4. The leading strand at a replication fork is synthesised:โœ“ Continuously toward the fork
Q5. In bacteria, the RNA primers laid down during replication are removed by:โœ“ DNA polymerase I
Q6. Of the 64 codons, the number that specify amino acids (sense codons) is:โœ“ 61
Q7. The codon that serves as the initiation (start) codon is:โœ“ AUG
Q8. The pairing between a codon on mRNA and the anticodon on tRNA during translation is part of:โœ“ Decoding (reading the message)
Q9. In Nirenberg's in-vitro experiments, a synthetic RNA of repeating cytosines (poly-C) directed the synthesis of a polypeptide of:โœ“ Proline
Q10. The main components of ribosomes are:โœ“ Ribosomal RNA (rRNA) and proteins
Q11. In bacteria, the Shine-Dalgarno sequence of mRNA base-pairs with the:โœ“ 16S rRNA (anti-Shine-Dalgarno)
Q12. In the lac operon, when both lactose and glucose are very low, the binding status of regulatory proteins is:โœ“ Both the repressor and CAP-cAMP are bound to their sites
Q13. Rho-independent (intrinsic) transcription termination in bacteria relies on:โœ“ A GC-rich RNA hairpin followed by a poly-U stretch (no protein factor)
Q14. During transcription, DNA gyrase helps by:โœ“ Introducing negative supercoils to relieve torsional strain
Q15. Protein splicing refers to the removal of:โœ“ An internal protein segment (intein), joining the flanking exteins
Q16. Which statement about the human mitochondrial genome is correct?โœ“ It has essentially no introns
Q17. The human mitochondrial genome contains a total of how many genes?โœ“ 37
Q18. The G+C content of a double-stranded DNA is 68%. The expected G+C content of an mRNA transcribed from it is approximately:โœ“ About 68%
Q19. LINEs (long interspersed elements) and SINEs (short interspersed elements) in the human genome are types of:โœ“ Mobile genetic elements (transposable elements)
Q20. Match each molecular feature with its description and select the correct option.โœ“ A-iii, B-iv, C-i, D-ii