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2.2 Eukaryotic Replication — Test 1
Q1. A principal difference between prokaryotic and eukaryotic DNA replication is:✓ Multiple origins in eukaryotes
Q2. Topoisomerase VI: choose the correct statements. I) Type II topoisomerase II) Type I topoisomerase III) present in all eukaryotes including humans IV) first identified in archaea.✓ I and IV
Q3. During eukaryotic DNA replication, which protein binds the newly separated parental strands generated by helicase activity?✓ RPA
Q4. Each origin of replication is activated only once per cell cycle because:✓ Pre-replicative complex forms only in G1 and replication initiates only when it is disassembled at the start of S-phase
Q5. Eukaryotic replication helicase (MCM) loading occurs during which phase?✓ G1 phase
Q6. In eukaryotic replication, priming and subsequent removal of the primer are catalysed respectively by:✓ Pol α and FEN1
Q7. Identify the correct statement about DNA gyrase:✓ Type II topoisomerase found in prokaryotes
Q8. In eukaryotes, lagging-strand replication is completed when:✓ Polymerase δ interacts with PCNA and RFC to complete the replication
Q9. A mammalian cell has 3 pg DNA and replicates in 6 hours; the nucleotide-addition rate is ~5% of E. coli's (E. coli genome 2×10⁹ Da, replicated in 40 min). Approximately how many replication origins must fire?✓ 16000
Q10. In some mammalian cells the nucleotide-addition rate is ~5% that of E. coli. Roughly how many replication origins must fire (standard textbook estimate)?✓ 16000
Q11. Which statement about eukaryotic leading- and lagging-strand synthesis is true?✓ The lagging strand needs primers synthesized by Pol α
Q12. Which DNA polymerase exhibits template-independent polymerase activity?✓ Terminal transferase
Q13. Which oligonucleotide is used as the first primer in reverse-transcriptase PCR for eukaryotic mRNA?✓ Oligo dT
Q14. Which statement about DNA replication in E. coli is NOT true?✓ Replication can begin at multiple origins simultaneously
Q15. The eukaryotic origin recognition complex (ORC) functions to:✓ Mark replication origins and nucleate pre-RC assembly
Q16. Autonomously replicating sequences (ARS) in yeast function as:✓ Origins of replication
Q17. PCNA enhances the processivity of replicative DNA polymerases by acting as a:✓ Sliding clamp encircling DNA
Q18. The end-replication problem of linear eukaryotic chromosomes is solved by:✓ Telomerase extending the 3' end
Q19. Replication licensing ensures the genome is copied once per cycle by restricting which step to G1?✓ Loading of the MCM helicase (pre-RC assembly)
Q20. Which eukaryotic DNA polymerase is primarily responsible for leading-strand synthesis?✓ Pol ε