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4.2 Eukaryotic Transcription β Test 1
Q1. A specific inhibitor of the kinase (phosphorylation) activity of TFIIH is added to an in-vitro transcription reaction. Which step is most affected?β Promoter clearance (escape into elongation)
Q2. Which technique can determine the transcription RATE of a gene (not just steady-state level)?β Global/nuclear run-on sequencing
Q3. In eukaryotes, the rRNA genes (45S precursor) are transcribed by:β RNA polymerase I
Q4. Which technique CANNOT precisely map the transcription start site?β Sequencing the region downstream of the promoter
Q5. In eukaryotes, the TATA box is typically located:β ~25β35 bp upstream of the transcription start site
Q6. The method that measures transcription rate of genes on a GLOBAL scale is:β GRO-Seq (global run-on)
Q7. The Kozak consensus sequence (around the start AUG) is:β (gcc)gccRccAUGG β classically ACCAUGG
Q8. Which eukaryotic RNA polymerase is essentially INSENSITIVE to Ξ±-amanitin?β RNA Pol I
Q9. The promoter for 5S rRNA (transcribed by Pol III) is located:β Internally, within the transcribed region (e.g. +55 to +80)
Q10. Which sequence module is part of the eukaryotic BASAL (core) promoter?β TATA box
Q11. The Ξ±-amanitin sensitivity order of eukaryotic RNA polymerases (mostβleast) is:β Pol II > Pol III > Pol I
Q12. Which transcription factor acts as the 'positioning factor' that binds the promoter (TATA box) for RNA Pol II?β TFIID (contains TBP)
Q13. The method used to determine genome-wide DNA-binding regions of a transcription factor is:β ChIP-Seq
Q14. Which protein is phosphorylated during transcription elongation by Pol II?β The CTD (C-terminal domain) of RNA Pol II
Q15. Which transcription factor phosphorylates the CTD of RNA Pol II during initiation?β TFIIH (CDK7 kinase)
4.2 Eukaryotic Transcription β Test 2
Q16. The promoters of RNA polymerase III (type I and II) are unusual because they are:β Located internal to the transcribed sequence
Q17. Transcribing 5'-AGCTGCAATG-3' (this strand as template-equivalent), the RNA produced (replacing T with U on the coding-strand sequence) is:β 5'-AGCUGCAAUG-3'
Q18. High-CpG-content promoters are predominantly found in:β Housekeeping genes
Q19. Regulatory elements controlling rRNA gene expression reside largely in the:β Non-transcribed spacer region
Q20. Which statement about eukaryotic gene expression is correct?β A single primary transcript can yield different mature mRNAs (alternative splicing)
Q21. Which eukaryotic rRNA is NOT processed from the single large (45S) transcription unit?β 5S
Q22. Which statement holds for eukaryotic transcription of most genes?β Discontinuous and stochastic (transcriptional bursting)
Q23. Which enzyme is NOT a eukaryotic RNA polymerase but assists transcription by relieving torsional stress?β Topoisomerase
Q24. RNA Pol II generally transcribes:β mRNAs and many small non-coding RNAs (snRNAs, miRNAs)
Q25. Eukaryotic mRNA, unlike prokaryotic, is characteristically:β Modified at both 5' (cap) and 3' (poly-A) ends
Q26. FACT, a histone chaperone, functions during transcription to:β Help displace/reassemble histone octamers ahead of and behind elongating Pol II
Q27. TBP (TATA-binding protein) is a subunit of which general transcription factor?β TFIID
Q28. The pre-initiation complex (PIC) for Pol II assembles in the order:β TFIID (TBP) binds TATA, then TFIIB, then Pol II/TFIIF, then TFIIE/TFIIH
Q29. Which assay maps open/accessible chromatin genome-wide?β ATAC-Seq
Q30. The physical looping that brings a distal enhancer close to a promoter is detected by:β Chromosome conformation capture (3C and derivatives)