Endospore Staining Procedure (Schaeffer Fulton Method)

Welcome to your Endospore Staining Procedure (Schaeffer Fulton Method)

What is the primary reason endospores resist standard staining techniques?

A. They lack a cell wall

B. They have a lipid-rich membrane

C. They possess a thick, keratin-like spore coat

D. They actively pump out the dye

During the Schaeffer-Fulton method, what role does heat play in the staining process?

A. It kills vegetative cells to reveal endospores

B. It evaporates excess stain

C. It softens the spore coat to allow dye penetration

D. It prevents counterstaining of vegetative cells

Why are 48–72 hour old cultures preferred for endospore staining?

A. They grow faster

B. They contain fewer contaminants

C. They contain more endospores due to nutrient limitation

D. They produce pigments that help staining

Which of the following stains is used as the primary dye in the Schaeffer-Fulton method?

A. Crystal violet

B. Methylene blue

C. Safranin

D. Malachite green

What color do vegetative cells appear after the complete Schaeffer-Fulton staining procedure?

A. Blue

B. Green

C. Colorless

D. Pink or Red

What would happen if the steaming step was skipped during the Schaeffer-Fulton method?

A. Endospores would stain darker

B. Vegetative cells would not stain

C. Endospores would not retain malachite green

D. Safranin would not bind to vegetative cells

Why is malachite green washed out from vegetative cells but retained in endospores?

A. Endospores are negatively charged

B. Vegetative cells bind dye more strongly

C. Malachite green is lipid-soluble

D. It binds weakly to cell components and is water-soluble

Which of the following best describes the Schaeffer-Fulton method?

A. Simple stain

B. Capsule stain

C. Negative stain

D. Differential stain

What is the function of safranin in the Schaeffer-Fulton staining method?

A. Stains endospores red

B. Acts as a mordant for malachite green

C. Counterstains vegetative cells pink/red

D. Fixes the smear on the slide

In microscope observation after the staining procedure, which of the following is a correct identification?

A. Green oval structures inside pink cells – Intracellular endospores

B. Pink round structures inside green cells – Free endospores

C. Green round structures inside pink cells – Capsule

D. Red oval structures outside cells – Endospore

Endospore Staining Procedure (Schaeffer Fulton Method)

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