Microorganisms in the genus Mycobacteria and Nocardia have complex cell walls with higher lipid content. The cell wall of these microbes contains mycolic acid, which is a complex hydrocarbon. Due to this waxy cell wall, bacteria don’t take up the stain easily. Therefore,…
Mannitol salt Agar is a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical samples. As the name suggests, Mannitol Salt Agar (MSA) contains two important components. First is mannitol, which is a…
It is a type of microbial culture medium. It is used to transport microbiological samples from one location to another. Microbiologists use transport medium to keep microbes in the sample or specimen alive when inoculation in growth media is delayed.…
Rappaport and Henig described a medium for isolation of enteropathogenic E coli serotype. Sorbitol MacConkey agar is also a type of medium based on formula Rappaport and Henig. It is selective and differential media. This media is for detection enterohaemorrhagic…
McConkey agar is a complex growth medium for culturing bacteria. MacConkey agar is selective and differential media. This medium is selective because, it selects and allows growth of only gram-negative bacteria. It is differential because it differentiates lactose fermenters and…
In microbiology, some bacteria are termed fastidious and non-fastidious. Fastidious bacteria require complex nutritional and environmental conditions to grow and survive. Fastidious microorganisms can not grow on routine culture media. Cultivation of fastidious bacteria in the laboratory require specific nutrient…
Culture media are the nutrient mediums for the cultivation of microorganisms. Culture media provide different nutrients for the growth and survival of bacteria. These culture media vary in physical consistency, use, and chemical composition. Nutrient agar and nutrient broth are…
Nutrient broth is a microbial culture medium. Microbial culture media without solidifying agents such as agar and gelatin are known as liquid media. Another name for liquid media is liquid broth. The liquid broth is used to grow bacteria in large…
DRDO is a premiere central government organization under the Ministry of Defence, Govt of India. There are many DRDO laboratories such as DFRL, DIPAS, INMAS, DRDE, and DIBER where R & D work is being done in the Life Science…
Differential staining procedures require more than one dye for staining. Two important differential staining procedures are Gram’s staining method and the acid-fast staining method. In the gram staining procedure, gram-positive cells stain purple in color, whereas the acid-fast staining procedure differentiates…