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Nutrient broth composition and uses

Nutrient broth is a microbial culture medium. Microbial culture media without solidifying agents such as agar and gelatin are known as liquid media. Another name for liquid media is liquid broth. The liquid broth is used to grow bacteria in large…

Acid fast staining method

Differential staining procedures require more than one dye for staining. Two important differential staining procedures are Gram’s staining method and the acid-fast staining method. In the gram staining procedure, gram-positive cells stain purple in color, whereas the acid-fast staining procedure differentiates…

Different types of staining in microbiology

types of staining

Microorganisms such as bacteria are very small, colorless, and transparent. As a result we cannot see these microorganisms with naked eyes. The reason why we can’t see microorganisms is that they lack contrast. In other words, without contrast and color,…

Operon in prokaryotes

Prokaryotes have special arrangement of genes on chromosome. This arrangement known as operon. However, operons are not found in eukaryotes. Operon is present only in prokaryotes. There is more than one gene in an operon. Genes present in an operon…

Lac operon

lac operon structure in e coli

Operons are set of genes which are present on chromosome of all prokaryotes. Lac operon is one such operon which helps E. coli to to metabolize lactose. In E. coli glucose is the primary carbon source for energy production. E…